|Calculated MW||54755 Da|
|Positive Control||3T3 cell lysate, rat kidney tissue lysate|
|Application & Usage||Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. 3T3 cell lysate can be used as a positive control.|
|Other Names||Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4|
|Formulation||200 µg (0.5 mg/ml) affinity purified rabbit anti-GLUT4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||GLUT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Insulin-regulated facilitative glucose transporter.|
|Cellular Location||Cell membrane; Multi-pass membrane protein Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region Note=Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration (By similarity).|
|Tissue Location||Skeletal and cardiac muscles; brown and white fat|
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Provided below are standard protocols that you may find useful for product applications.
Insulin stimulates glucose uptake in the muscles and adipose tissues by activating GLUT4 (Glucose Transporter Type 4). GLUT 4 is a transmembrane protein that facilitates glucose transport from storage sites to cell surface. Human insulin resistance involves a defect in GLUT4 that leads to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
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