|Application ||WB, IF, IP|
|Calculated MW||48804 Da|
|Application & Usage||Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.|
|Other Names||MYC , C-MYC , c-Myc|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||C-Myc Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).|
|Cellular Location||Nucleus, nucleoplasm. Nucleus, nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
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