|Calculated MW||133994 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). Recombinant human EGF can be used as a positive control. Per customer’s feedback, it can also be used in Neutralization and Elisa. However, the optimal conditions should be determined individually.|
|Other Names||Epidermal Growth Factor|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human EGF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in kidney, salivary gland, cerebrum and prostate.|
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Provided below are standard protocols that you may find useful for product applications.
EGF (Epidermal Growth Factor) is polypeptide growth factor that stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
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