|Application ||WB, E|
|Calculated MW||30770 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||FGF2, FGF-2, FGF 2, Fibroblast Growth Factor 2, FGF 2, FGF2, Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, bFGF, Putative heparin-binding growth factor 2, Kidney-derived growth factor|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human FGF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||FGF-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677).|
|Cellular Location||Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57|
|Tissue Location||Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.|
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Provided below are standard protocols that you may find useful for product applications.
FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Human FGF-2 is a 17.2 kDa protein containing 154 amino acid residues.
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