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S- Adenosylmethionine Antibody (Clone # 118-18)

Mouse Monoclonal Antibody

  • E - S- Adenosylmethionine Antibody (Clone # 118-18) ABV11453-25
    Competitive ELISA: 0.1 g/ml of SAM coating standard was coated into 96 wells. Serial dilution of SAM standard,S-Adenosylhomocysteine (SAH), Adenosine, L-Methionine and antibody were added. HRP conjugated Goat anti-Mouse IgG antibody was used to develop the color. The A is the OD450 value of the test well and the A0 is the OD450 of the well without competitive antigen.
  • IHC - S- Adenosylmethionine Antibody (Clone # 118-18) ABV11453-25
    Immunohistochemistry staining was performed using the antibody with benign breast tissue adjacent to carcinoma. Brown areas indicated strong positive staining in cytoplasm (X400).
  • IHC - S- Adenosylmethionine Antibody (Clone # 118-18) ABV11453-25
    The immunohistochemical staining was performed for the same sample as in the above figure with breast cancer tissue.Cytoplasm showed background staining (further dilution beyond 1:200 is required) with the antibody (X400).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity All Species
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 118-18
Additional Information
Positive Control IHC: breast carcinoma tissue, FCM: HepG2 and L02 cell lines
Application & Usage cELISA: 1:4000 – 1:15000, FCM: 1: 400, IHC: 1: 400.
Other Names S- Adenosylmethionine
Target/Specificity S Adenosylmethionine
Antibody Form Liquid
Appearance Colorless liquid
Formulation 20 mM PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide, 50% Glycerol and 10 mg/ml BSA
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsS- Adenosylmethionine Antibody (Clone # 118-18) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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S-Adenosylmethionine (SAM) is a naturally occurring compound that is found in almost every tissue and fluid in the body. It is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyl transferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. SAM plays a role in the immune system, maintains cell membranes, and helps produce and break down brain chemicals, such as serotonin, melatonin, and dopamine. It works with vitamin B12 and folate (vitamin B9). Being deficient in either vitamin B12 or folate may reduce levels of SAM in your body.

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$ 165.00
Cat# ABV11453-25
Availability: 5-7days
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