|Application ||WB, IHC|
|Calculated MW||97456 Da|
|Positive Control||WB: MCF-7 cells; IHC: human breast cancer tissues|
|Application & Usage||IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution|
|Other Names||P-cadherin, N-Cadherin, E-Cadherin, K-Cadherin, M-jadherin, R-Cadherin|
|Formulation||In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide|
|Reconstitution & Storage||-20 °C|
|Precautions||Anti-E-Cadherin (CDH1) Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.|
|Cellular Location||Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane|
|Tissue Location||Non-neural epithelial tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Cadherins comprise a family of Ca-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal domains. The relative short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function.
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