|Application ||WB, IH|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||32745 Da|
|Positive Control||WB: HeLa, HuvEc, DKD, mouse brain, rat brain lysates ; IHC: human ovarian cancer tissue|
|Application & Usage||WB; 1:500 – 1:2000, IH; 1:50 – 1:200|
|Other Names||CAF1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Anti-CNOT7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti- proliferative activity.|
|Cellular Location||Nucleus. Cytoplasm, P-body. Note=NANOS2 promotes its localization to P-body.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.