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Functional Neutrophils (mouse) Antibody, mAb (blocking) (Biotin)

  • FC - Functional Neutrophils (mouse) Antibody, mAb (blocking) (Biotin) ADP0032
    Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14).
    Method:Mice were injected) i.p. with 250 µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to Leishmania major infection (3x1000000 parasites injected in the hind footpad). 3 days later, blood (100 µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Mouse
Host Purified From Concentrated Hybridoma Tissue Culture Supernatant.
Clonality Monoclonal
Isotype Rat IgG2b
Gene Source Mouse
Endotoxin <0.1EU/ µg purified protein (LAL test; Lonza).
Application Note FC(1:100),IHC-F(1:200),IHC-P(1:200),ICC(1:200),Functional Applicational (Blocking), 250 µg / mouse (Optimal reagent to deplete neutrophils in vivo,)
Clone Names Nimp-R14
Description Negative control: AG-35B-0011
Additional Information
Target/Specificity Recognizes mouse neutrophils (surface protein mouse Ly-6G).
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional Neutrophils (mouse) Antibody, mAb (blocking) (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
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Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.

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$ 290.00
Cat# ADP0032
Availability: 3-5 days
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