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Goat Anti-Acylglycerol kinase Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-Acylglycerol kinase Antibody AF1023a
    AF1023a (1 µg/ml) staining of Human Brain (Substantia nigra) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q53H12
Other Accession NP_060708, 55750, 69923 (mouse)
Reactivity Human
Predicted Mouse
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 47137 Da
Additional Information
Gene ID 55750
Other Names Acylglycerol kinase, mitochondrial, hAGK, 2.7.1.107, 2.7.1.94, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase, AGK, MULK
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Acylglycerol kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGK {ECO:0000303|PubMed:15939762, ECO:0000312|HGNC:HGNC:21869}
Function Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15939762). Does not phosphorylate sphingosine (PubMed:15939762). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed:28712724, PubMed:28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed:28712724, PubMed:28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed:28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed:15939762).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein Mitochondrion intermembrane space. Note=Localizes in the mitochondrion intermembrane space, where it associates with the inner membrane (PubMed:28712724). It is unclear whether the N-terminal hydrophobic region forms a transmembrane region or associates with the membrane without crossing it (PubMed:28712724, PubMed:28712726).
Tissue Location Highly expressed in muscle, heart, kidney and brain.
Research Areas
Citations (0)

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References

Expression of autotaxin and acylglycerol kinase in prostate cancer: association with cancer development and progression. Nouh MA, et al. Cancer Sci, 2009 Sep. PMID 19549252.
Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase. Epand RM, et al. Biochemistry, 2007 Dec 11. PMID 18004883.
Functions of the multifaceted family of sphingosine kinases and some close relatives. Spiegel S, et al. J Biol Chem, 2007 Jan 26. PMID 17135245.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies. Van Overloop H, et al. J Lipid Res, 2006 Feb. PMID 16269826.

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$ 368.00
Cat# AF1023a
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