|Application ||WB, E|
|Other Accession||NP_036228, 26060, 72993 (mouse)|
|Predicted||Human, Mouse, Rat, Dog, Cow|
|Calculated MW||79663 Da|
|Other Names||DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-APPL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.|
|Cellular Location||Early endosome membrane; Peripheral membrane protein. Nucleus. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF|
|Tissue Location||High levels in heart, ovary, pancreas and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Rab5a overexpression promoting ovarian cancer cell proliferation may be associated with APPL1-related epidermal growth factor signaling pathway. Zhao Z, et al. Cancer Sci, 2010 Jun. PMID 20412119.
Identification of phosphorylation sites within the signaling adaptor APPL1 by mass spectrometry. Gant-Branum RL, et al. J Proteome Res, 2010 Mar 5. PMID 20095645.
Functional characterization of the interactions between endosomal adaptor protein APPL1 and the NuRD co-repressor complex. Banach-Orlowska M, et al. Biochem J, 2009 Oct 12. PMID 19686092.
Adiponectin activates AMP-activated protein kinase in muscle cells via APPL1/LKB1-dependent and phospholipase C/Ca2+/Ca2+/calmodulin-dependent protein kinase kinase-dependent pathways. Zhou L, et al. J Biol Chem, 2009 Aug 14. PMID 19520843.
Endosomal adaptor proteins APPL1 and APPL2 are novel activators of beta-catenin/TCF-mediated transcription. Rashid S, et al. J Biol Chem, 2009 Jul 3. PMID 19433865.
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