|Application ||WB, IHC, E|
|Other Accession||NP_055647, 9882|
|Calculated MW||146563 Da|
|Other Names||TBC1 domain family member 4, Akt substrate of 160 kDa, AS160, TBC1D4, AS160, KIAA0603|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-AS160 / TBC1D4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.|
|Cellular Location||Cytoplasm. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells|
|Tissue Location||Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle. Treebak JT, et al. Am J Physiol Cell Physiol, 2010 Feb. PMID 19923418.
A truncation mutation in TBC1D4 in a family with acanthosis nigricans and postprandial hyperinsulinemia. Dash S, et al. Proc Natl Acad Sci U S A, 2009 Jun 9. PMID 19470471.
Potential role of TBC1D4 in enhanced post-exercise insulin action in human skeletal muscle. Treebak JT, et al. Diabetologia, 2009 May. PMID 19252894.
Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Ishibashi K, et al. Genes Cells, 2009 Jan. PMID 19077034.
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