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Goat Anti-CMG1 / CCDC2 / IFT74 Antibody

Peptide-affinity purified goat antibody

  • IF - Goat Anti-CMG1 / CCDC2 / IFT74 Antibody AF1257a
    A) AF1257a (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
  • WB - Goat Anti-CMG1 / CCDC2 / IFT74 Antibody AF1257a
    AF1257a staining (0.3 µg/ml) of 293 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-CMG1 / CCDC2 / IFT74 Antibody AF1257a
    AF1257a (5 µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: AF1257a (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96LB3
Other Accession NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1, 80173, 313365 (rat)
Reactivity Human, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 69239 Da
Additional Information
Gene ID 80173
Other Names Intraflagellar transport protein 74 homolog, Capillary morphogenesis gene 1 protein, CMG-1, Coiled-coil domain-containing protein 2, IFT74, CCDC2, CMG1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CMG1 / CCDC2 / IFT74 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFT74
Synonyms CCDC2, CMG1
Function Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis.
Cellular Location Cell projection, cilium. Cytoplasmic vesicle. Note=Localizes along primary cilia at interphase and around the basal body/centriole at interphase and mitosis
Tissue Location Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.
Research Areas
Citations (0)

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Genetic studies of GRN and IFT74 in amyotrophic lateral sclerosis. Xiao S, et al. Neurobiol Aging, 2008 Aug. PMID 17383054.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.

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$ 368.00
Cat# AF1257a
Availability: 7-10 days
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