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Goat Anti-CRP2 / CSRP2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-CRP2 / CSRP2 Antibody AF1278a
    AF1278a (0.1 µg/ml) staining of mouse kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - Goat Anti-CRP2 / CSRP2 Antibody AF1278a
    HEK293 overexpressing CRP2 (RC201565) and probed with AF1278a (mock transfection in first lane), tested by Origene.
  • IHC - Goat Anti-CRP2 / CSRP2 Antibody AF1278a
    AF1278a (3.8 µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16527
Other Accession NP_001312, 1466, 13008 (mouse)
Reactivity Human, Mouse, Rat
Predicted Horse
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Additional Information
Other Names Cysteine and glycine-rich protein 2, Cysteine-rich protein 2, CRP2, LIM domain only protein 5, LMO-5, Smooth muscle cell LIM protein, SmLIM, CSRP2, LMO5, SMLIM
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CRP2 / CSRP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSRP2
Synonyms LMO5, SMLIM
Function Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system.
Cellular Location Nucleus.
Tissue Location Highly expressed in the aorta, but not in heart and skeletal muscle EMBL; U46006; AAC27344.1; -; mRNA EMBL; U57646; AAC51753.1; -; mRNA EMBL; U95018; AAC51755.1; -; Genomic_DNA EMBL; BT019913; AAV38716.1; -; mRNA EMBL; BC000992; AAH00992.1; -; mRNA CCDS; CCDS9015.1; - RefSeq; NP_001287894.1; NM_001300965.1 RefSeq; NP_001312.1; NM_001321.2 UniGene; Hs.530904; - ProteinModelPortal; Q16527; - BioGrid; 107848; 17 IntAct; Q16527; 17 MINT; Q16527; - STRING; 9606.ENSP00000310901; - iPTMnet; Q16527; - PhosphoSitePlus; Q16527; - BioMuta; CSRP2; - DMDM; 2497674; - EPD; Q16527; - jPOST; Q16527; - MaxQB; Q16527; - PaxDb; Q16527; - PeptideAtlas; Q16527; - PRIDE; Q16527; - ProteomicsDB; 60894; - DNASU; 1466; - Ensembl; ENST00000311083; ENSP00000310901; ENSG00000175183 Ensembl; ENST00000546966; ENSP00000450056; ENSG00000175183 GeneID; 1466; - KEGG; hsa:1466; - UCSC; uc001syl.2; human CTD; 1466; - DisGeNET; 1466; - EuPathDB; HostDB:ENSG00000175183.9; - GeneCards; CSRP2; - HGNC; HGNC:2470; CSRP2 HPA; HPA045617; - MIM; 601871; gene neXtProt; NX_Q16527; - OpenTargets; ENSG00000175183; - PharmGKB; PA26968; - eggNOG; ENOG410ITHU; Eukaryota eggNOG; ENOG41128N9; LUCA GeneTree; ENSGT00940000154980; - HOGENOM; HOG000111233; - HOVERGEN; HBG051143; - InParanoid; Q16527; - KO; K09377; - OrthoDB; 1214165at2759; - PhylomeDB; Q16527; - TreeFam; TF313758; - GeneWiki; CSRP2; - GenomeRNAi; 1466; - PRO; PR:Q16527; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000175183; Expressed in 231 organ(s), highest expression level in frontal cortex ExpressionAtlas; Q16527; baseline and differential Genevisible; Q16527; HS GO; GO:0005925; C:focal adhesion; HDA:UniProtKB GO; GO:0005634; C:nucleus; NAS:UniProtKB GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0030154; P:cell differentiation; NAS:UniProtKB GO; GO:0007275; P:multicellular organism development; IEA:UniProtKB-KW InterPro; IPR001781; Znf_LIM Pfam; PF00412; LIM; 2 SMART; SM00132; LIM; 2 PROSITE; PS00478; LIM_DOMAIN_1; 2 PROSITE; PS50023; LIM_DOMAIN_2; 2 1: Evidence at protein level; Acetylation; Complete proteome; Developmental protein; Differentiation; Isopeptide bond; LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat; Ubl conjugation; Zinc CHAIN 1 193 Cysteine and glycine-rich protein 2 /FTId=PRO_0000075721 DOMAIN 10 61 LIM zinc-binding 1. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 119 170 LIM zinc-binding 2. {ECO:0000255|PROSITE- ProRule:PRU00125} MOTIF 64 69 Nuclear localization signal COMPBIAS 63 78 Gly-rich COMPBIAS 176 187 Gly-rich MOD_RES 112 112 N6-acetyllysine {ECO:0000250|UniProtKB:P97314} MOD_RES 131 131 N6-acetyllysine {ECO:0000250|UniProtKB:P21291} MOD_RES 137 137 N6-acetyllysine; alternate {ECO:0000250|UniProtKB:P97315} MOD_RES 137 137 N6-succinyllysine; alternate {ECO:0000250|UniProtKB:P97314} MOD_RES 161 161 N6-acetyllysine {ECO:0000250|UniProtKB:P97315} CROSSLNK 91 91 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) SEQUENCE 193 AA; 20954 MW; 2E4231D6427579E7 CRC64; MPVWGGGNKC GACGRTVYHA EEVQCDGRSF HRCCFLCMVC RKNLDSTTVA IHDEEIYCKS CYGKKYGPKG YGYGQGAGTL NMDRGERLGI KPESVQPHRP TTNPNTSKFA QKYGGAEKCS RCGDSVYAAE KIIGAGKPWH KNCFRCAKCG KSLESTTLTE KEGEIYCKGC YAKNFGPKGF GYGQGAGALV HAQ
Research Areas
Citations (0)

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CSRP2 is a member of the CSRP family of genes, encoding a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity, and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3.


The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Interleukin-10 activation of the interleukin-10E1 pathway and tissue inhibitor of metalloproteinase-1 expression is enhanced by proteasome inhibitors in primary prostate tumor lines. Stearns ME, et al. Mol Cancer Res, 2003 Jul. PMID 12861049.
IL-10/IL-10 receptor signaling regulates TIMP-1 expression in primary human prostate tumor lines. Wang M, et al. Cancer Biol Ther, 2002 Sep-Oct. PMID 12496489.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.
LIM-domain protein cysteine- and glycine-rich protein 2 (CRP2) is a novel marker of hepatic stellate cells and binding partner of the protein inhibitor of activated STAT1. Weiskirchen R, et al. Biochem J, 2001 Nov 1. PMID 11672422.

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$ 368.00
Cat# AF1278a
Availability: 7-10 days
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