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Goat Anti-CYB561D2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-CYB561D2 Antibody AF1287a
    AF1287a staining (1 µg/ml) of human liver lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14569
Other Accession NP_008953, 11068, 56368 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Additional Information
Other Names Cytochrome b561 domain-containing protein 2, Putative tumor suppressor protein 101F6, CYB561D2, 101F6
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CYB561D2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB561D2 (HGNC:30253)
Function Two-heme-containing cytochrome that catalyzes ascorbate- dependent trans-membrane ferric-chelate reduction.
Cellular Location Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q9WUE3}; Multi-pass membrane protein. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9WUE3}; Multi-pass membrane protein EMBL; AF040704; AAC70911.1; -; mRNA EMBL; AK291167; BAF83856.1; -; mRNA EMBL; AC002481; AAB67309.1; -; Genomic_DNA EMBL; CH471055; EAW65113.1; -; Genomic_DNA EMBL; BC047691; AAH47691.1; -; mRNA CCDS; CCDS2827.1; - RefSeq; NP_001278213.1; NM_001291284.1 RefSeq; NP_008953.1; NM_007022.4 UniGene; Hs.149443; - UniGene; Hs.669905; - ProteinModelPortal; O14569; - SMR; O14569; - BioGrid; 116252; 4 IntAct; O14569; 19 STRING; 9606.ENSP00000232508; - iPTMnet; O14569; - PhosphoSitePlus; O14569; - BioMuta; CYB561D2; - EPD; O14569; - jPOST; O14569; - MaxQB; O14569; - PaxDb; O14569; - PeptideAtlas; O14569; - PRIDE; O14569; - ProteomicsDB; 48090; - DNASU; 11068; - Ensembl; ENST00000232508; ENSP00000232508; ENSG00000114395 Ensembl; ENST00000418577; ENSP00000391209; ENSG00000114395 Ensembl; ENST00000424512; ENSP00000410663; ENSG00000114395 Ensembl; ENST00000425346; ENSP00000400454; ENSG00000114395 GeneID; 11068; - KEGG; hsa:11068; - UCSC; uc003dal.4; human CTD; 11068; - DisGeNET; 11068; - EuPathDB; HostDB:ENSG00000114395.10; - GeneCards; CYB561D2; - HGNC; HGNC:30253; CYB561D2 HPA; HPA042817; - MIM; 607068; gene neXtProt; NX_O14569; - OpenTargets; ENSG00000114395; - PharmGKB; PA134977950; - eggNOG; ENOG410IVNX; Eukaryota eggNOG; ENOG4111KF1; LUCA GeneTree; ENSGT00440000038072; - HOGENOM; HOG000008685; - HOVERGEN; HBG050754; - InParanoid; O14569; - KO; K08371; - OMA; AKLKRYH; - OrthoDB; 1586923at2759; - PhylomeDB; O14569; - TreeFam; TF323584; - ChiTaRS; CYB561D2; human GenomeRNAi; 11068; - PRO; PR:O14569; - Proteomes; UP000005640; Chromosome 3 Bgee; ENSG00000114395; Expressed in 180 organ(s), highest expression level in right lobe of liver ExpressionAtlas; O14569; baseline and differential Genevisible; O14569; HS GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0031982; C:vesicle; ISS:UniProtKB GO; GO:0004322; F:ferroxidase activity; ISS:UniProtKB GO; GO:0020037; F:heme binding; IDA:UniProtKB GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW InterPro; IPR006593; Cyt_b561/ferric_Rdtase_TM Pfam; PF03188; Cytochrom_B561; 1 SMART; SM00665; B561; 1 PROSITE; PS50939; CYTOCHROME_B561; 1 1: Evidence at protein level; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Electron transport; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Reference proteome; Translocase; Transmembrane; Transmembrane helix; Transport INIT_MET 1 1 Removed. CHAIN 2 222 Cytochrome b561 domain-containing protein 2 /FTId=PRO_0000151036 TRANSMEM 18 38 Helical. TRANSMEM 47 67 Helical. TRANSMEM 86 106 Helical. TRANSMEM 123 143 Helical. TRANSMEM 163 183 Helical. TRANSMEM 187 207 Helical. DOMAIN 14 217 Cytochrome b561. {ECO:0000255|PROSITE- ProRule:PRU00242} METAL 48 48 Iron (heme axial ligand) {ECO:0000255|PROSITE-ProRule:PRU00242} METAL 86 86 Iron (heme axial ligand) {ECO:0000255|PROSITE-ProRule:PRU00242} METAL 120 120 Iron (heme axial ligand) {ECO:0000255|PROSITE-ProRule:PRU00242} METAL 159 159 Iron (heme axial ligand) {ECO:0000255|PROSITE-ProRule:PRU00242} SEQUENCE 222 AA; 23974 MW; 3AEEE64D8CB91A32 CRC64; MALSAETESH IYRALRTASG AAAHLVALGF TIFVAVLARP GSSLFSWHPV LMSLAFSFLM TEALLVFSPE SSLLHSLSRK GRARCHWVLQ LLALLCALLG LGLVILHKEQ LGKAHLVTRH GQAGLLAVLW AGLQCSGGVG LLYPKLLPRW PLAKLKLYHA TSGLVGYLLG SASLLLGMCS LWFTASVTGA AWYLAVLCPV LTSLVIMNQV SNAYLYRKRI QP
Research Areas
Citations (0)

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Functional expression and characterization of human 101F6 protein, a homologue of cytochrome b561 and a candidate tumor suppressor gene product. Recuenco MC, et al. Biofactors, 2009. PMID 19734123.
The DNA sequence, annotation and analysis of human chromosome 3. Muzny DM, et al. Nature, 2006 Apr 27. PMID 16641997.
A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.

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$ 368.00
Cat# AF1287a
Availability: 7-10 days
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