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Goat Anti-HMG20A Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-HMG20A Antibody AF1531a
    AF1531a (2.5 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NP66
Other Accession NP_060670, 10363, 66867 (mouse)
Reactivity Human, Rat
Predicted Mouse, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Additional Information
Other Names High mobility group protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1, HMG domain-containing protein HMGX1, HMG20A, HMGX1, HMGXB1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-HMG20A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMG20A
Synonyms HMGX1, HMGXB1
Function Plays a role in neuronal differentiation as chromatin- associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4 (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}
Tissue Location Ubiquitous.. EMBL; AF146222; AAF66706.1; -; mRNA EMBL; AL355736; CAB90815.1; ALT_TERM; mRNA EMBL; AL355737; CAB90816.1; -; mRNA EMBL; AK001601; BAA91782.1; -; mRNA EMBL; BT006716; AAP35362.1; -; mRNA EMBL; AK223100; BAD96820.1; -; mRNA EMBL; AC090984; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471136; EAW99201.1; -; Genomic_DNA EMBL; CH471136; EAW99202.1; -; Genomic_DNA EMBL; CH471136; EAW99203.1; -; Genomic_DNA EMBL; BC021959; AAH21959.1; -; mRNA CCDS; CCDS10295.1; -. [Q9NP66-1] RefSeq; NP_001291433.1; NM_001304504.1. [Q9NP66-1] RefSeq; NP_001291434.1; NM_001304505.1 RefSeq; NP_060670.1; NM_018200.3. [Q9NP66-1] RefSeq; XP_011519460.1; XM_011521158.2. [Q9NP66-1] UniGene; Hs.69594; - ProteinModelPortal; Q9NP66; - SMR; Q9NP66; - BioGrid; 115643; 109 CORUM; Q9NP66; - IntAct; Q9NP66; 86 MINT; Q9NP66; - STRING; 9606.ENSP00000336856; - iPTMnet; Q9NP66; - PhosphoSitePlus; Q9NP66; - BioMuta; HMG20A; - DMDM; 74734297; - EPD; Q9NP66; - jPOST; Q9NP66; - MaxQB; Q9NP66; - PaxDb; Q9NP66; - PeptideAtlas; Q9NP66; - PRIDE; Q9NP66; - ProteomicsDB; 81896; - ProteomicsDB; 81897; -. [Q9NP66-2] DNASU; 10363; - Ensembl; ENST00000336216; ENSP00000336856; ENSG00000140382. [Q9NP66-1] Ensembl; ENST00000381714; ENSP00000371133; ENSG00000140382. [Q9NP66-1] GeneID; 10363; - KEGG; hsa:10363; - UCSC; uc002bcr.4; human. [Q9NP66-1] CTD; 10363; - DisGeNET; 10363; - EuPathDB; HostDB:ENSG00000140382.14; - GeneCards; HMG20A; - HGNC; HGNC:5001; HMG20A HPA; HPA008126; - MIM; 605534; gene neXtProt; NX_Q9NP66; - OpenTargets; ENSG00000140382; - PharmGKB; PA29331; - eggNOG; KOG0381; Eukaryota eggNOG; COG5648; LUCA GeneTree; ENSGT00940000158464; - HOGENOM; HOG000247029; - HOVERGEN; HBG059870; - InParanoid; Q9NP66; - OMA; AQDRQKG; - OrthoDB; 1458939at2759; - PhylomeDB; Q9NP66; - TreeFam; TF106440; - ChiTaRS; HMG20A; human GeneWiki; HMG20A; - GenomeRNAi; 10363; - PRO; PR:Q9NP66; - Proteomes; UP000005640; Chromosome 15 Bgee; ENSG00000140382; Expressed in 226 organ(s), highest expression level in frontal cortex ExpressionAtlas; Q9NP66; baseline and differential Genevisible; Q9NP66; HS GO; GO:0005634; C:nucleus; NAS:UniProtKB GO; GO:0003677; F:DNA binding; NAS:UniProtKB GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB GO; GO:0042802; F:identical protein binding; IPI:IntAct GO; GO:0046982; F:protein heterodimerization activity; IEA:Ensembl GO; GO:0006325; P:chromatin organization; NAS:UniProtKB GO; GO:0045665; P:negative regulation of neuron differentiation; IEA:Ensembl GO; GO:0033234; P:negative regulation of protein sumoylation; IEA:Ensembl GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB Gene3D;; -; 1 InterPro; IPR009071; HMG_box_dom InterPro; IPR036910; HMG_box_dom_sf Pfam; PF00505; HMG_box; 1 SMART; SM00398; HMG; 1 SUPFAM; SSF47095; SSF47095; 1 PROSITE; PS50118; HMG_BOX_2; 1 1: Evidence at protein level; Alternative splicing; Chromatin regulator; Coiled coil; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation CHAIN 1 347 High mobility group protein 20A /FTId=PRO_0000238649 DNA_BIND 103 171 HMG box. {ECO:0000255|PROSITE- ProRule:PRU00267} COILED 229 273 MOD_RES 105 105 Phosphoserine VAR_SEQ 80 81 QR -> VS (in isoform 2) {ECO:0000303|Ref.2} /FTId=VSP_018621 VAR_SEQ 82 347 Missing (in isoform 2) {ECO:0000303|Ref.2} /FTId=VSP_018622 CONFLICT 320 320 L -> P (in Ref. 5; BAD96820) SEQUENCE 347 AA; 40144 MW; D8632FFAAA162587 CRC64; MENLMTSSTL PPLFADEDGS KESNDLATTG LNHPEVPYSS GATSSTNNPE FVEDLSQGQL LQSESSNAAE GNEQRHEDEQ RSKRGGWSKG RKRKKPLRDS NAPKSPLTGY VRFMNERREQ LRAKRPEVPF PEITRMLGNE WSKLPPEEKQ RYLDEADRDK ERYMKELEQY QKTEAYKVFS RKTQDRQKGK SHRQDAARQA THDHEKETEV KERSVFDIPI FTEEFLNHSK AREAELRQLR KSNMEFEERN AALQKHVESM RTAVEKLEVD VIQERSRNTV LQQHLETLRQ VLTSSFASMP LPGSGETPTV DTIDSYMNRL HSIILANPQD NENFIATVRE VVNRLDR
Research Areas
Citations (0)

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Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Functional proteomics mapping of a human signaling pathway. Colland F, et al. Genome Res, 2004 Jul. PMID 15231748.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.

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$ 368.00
Cat# AF1531a
Availability: 7-10 days
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