|Application ||WB, E|
|Other Accession||NP_002154, 3394, 15900 (mouse)|
|Predicted||Mouse, Rat, Cow|
|Calculated MW||48356 Da|
|Other Names||Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, H-ICSBP, ICSBP, IRF8, ICSBP1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ICSBP1 / IRF8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role as a transcriptional activator or repressor (PubMed:25122610). Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Positively regulates macroautophagy in dendritic cells (PubMed:29434592).|
|Cellular Location||Nucleus. Cytoplasm. Note=In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN- gamma induction.|
|Tissue Location||Predominantly expressed in lymphoid tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
The combination of gene perturbation assay and ChIP-chip reveals functional direct target genes for IRF8 in THP-1 cells. Kubosaki A, et al. Mol Immunol, 2010 Aug. PMID 20573402.
Activation of IL-27 p28 gene transcription by interferon regulatory factor 8 in cooperation with interferon regulatory factor 1. Zhang J, et al. J Biol Chem, 2010 Jul 9. PMID 20435892.
Validation of the CD6 and TNFRSF1A loci as risk factors for multiple sclerosis in Spain. Swaminathan B, et al. J Neuroimmunol, 2010 Jun. PMID 20430450.
Human variation in alcohol response is influenced by variation in neuronal signaling genes. Joslyn G, et al. Alcohol Clin Exp Res, 2010 May. PMID 20201926.
Common variants at 2q37.3, 8q24.21, 15q21.3 and 16q24.1 influence chronic lymphocytic leukemia risk. Crowther-Swanepoel D, et al. Nat Genet, 2010 Feb. PMID 20062064.
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