|Application ||WB, IHC, E|
|Other Accession||NP_000623, 3684, 16409 (mouse), 25021 (rat)|
|Predicted||Mouse, Rat, Dog|
|Calculated MW||127179 Da|
|Other Names||Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ITGAM / CD11B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177- PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein|
|Tissue Location||Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
ITGAM Arg77His Is Associated with Disease Susceptibility, Arthritis, and Renal Symptoms in Systemic Lupus Erythematosus Patients from a Sample of the Polish Population. Warcho? T, et al. DNA Cell Biol, 2010 Jul 28. PMID 20666624.
Association of ITGAM polymorphism with systemic lupus erythematosus: a meta-analysis. Fan Y, et al. J Eur Acad Dermatol Venereol, 2010 Jul 13. PMID 20629846.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Urokinase receptor (uPAR) regulates complement receptor 3 (CR3)-mediated neutrophil phagocytosis. Pliyev BK, et al. Biochem Biophys Res Commun, 2010 Jun 25. PMID 20580686.
Short-term IL-1beta blockade reduces monocyte CD11b integrin expression in an IL-8 dependent fashion in patients with type 1 diabetes. Sanda S, et al. Clin Immunol, 2010 Aug. PMID 20483667.
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