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Goat Anti-MAD4 / MXD4 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-MAD4 / MXD4 Antibody AF1645a
    AF1645a staining (0.5 µg/ml) of human kidney lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14582
Other Accession NP_006445, 10608
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Additional Information
Other Names Max dimerization protein 4, Max dimerizer 4, Class C basic helix-loop-helix protein 12, bHLHc12, Max-associated protein 4, Max-interacting transcriptional repressor MAD4, MXD4, BHLHC12, MAD4
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-MAD4 / MXD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MXD4
Synonyms BHLHC12, MAD4
Function Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981} EMBL; AF040963; AAB97009.1; -; mRNA EMBL; AF082740; AAF98553.1; -; mRNA EMBL; BT019969; AAV38772.1; -; mRNA EMBL; BT019970; AAV38773.1; -; mRNA EMBL; Z49250; CAA89217.1; -; Genomic_DNA EMBL; AL158068; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC068060; AAH68060.1; -; mRNA CCDS; CCDS3361.1; - RefSeq; NP_006445.1; NM_006454.2 UniGene; Hs.655020; - ProteinModelPortal; Q14582; - SMR; Q14582; - BioGrid; 115854; 8 ELM; Q14582; - IntAct; Q14582; 4 MINT; Q14582; - STRING; 9606.ENSP00000337889; - iPTMnet; Q14582; - PhosphoSitePlus; Q14582; - BioMuta; MXD4; - DMDM; 6016524; - PaxDb; Q14582; - PeptideAtlas; Q14582; - PRIDE; Q14582; - ProteomicsDB; 60056; - TopDownProteomics; Q14582; - DNASU; 10608; - Ensembl; ENST00000337190; ENSP00000337889; ENSG00000123933 GeneID; 10608; - KEGG; hsa:10608; - UCSC; uc003geu.2; human CTD; 10608; - DisGeNET; 10608; - EuPathDB; HostDB:ENSG00000123933.14; - GeneCards; MXD4; - HGNC; HGNC:13906; MXD4 HPA; HPA053363; - neXtProt; NX_Q14582; - OpenTargets; ENSG00000123933; - PharmGKB; PA134914966; - eggNOG; KOG2483; Eukaryota eggNOG; ENOG4111V1C; LUCA GeneTree; ENSGT00940000160120; - HOVERGEN; HBG006314; - InParanoid; Q14582; - KO; K09114; - OMA; RLQPHHC; - PhylomeDB; Q14582; - TreeFam; TF315654; - SIGNOR; Q14582; - ChiTaRS; MXD4; human GeneWiki; MXD4; - GenomeRNAi; 10608; - PRO; PR:Q14582; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000123933; Expressed in 198 organ(s), highest expression level in right hemisphere of cerebellum ExpressionAtlas; Q14582; baseline and differential Genevisible; Q14582; HS GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB GO; GO:0046983; F:protein dimerization activity; IEA:InterPro GO; GO:0003714; F:transcription corepressor activity; TAS:ProtInc GO; GO:0008285; P:negative regulation of cell population proliferation; TAS:ProtInc GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; TAS:ProtInc CDD; cd00083; HLH; 1 Gene3D;; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 1: Evidence at protein level; Complete proteome; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation CHAIN 1 209 Max dimerization protein 4 /FTId=PRO_0000127266 DOMAIN 53 105 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} REGION 6 23 Interaction with SIN3A and SIN3B SEQUENCE 209 AA; 23528 MW; ACFAFB44EB0FE7A2 CRC64; MELNSLLILL EAAEYLERRD REAEHGYASV LPFDGDFARE KTKAAGLVRK APNNRSSHNE LEKHRRAKLR LYLEQLKQLV PLGPDSTRHT TLSLLKRAKV HIKKLEEQDR RALSIKEQLQ QEHRFLKRRL EQLSVQSVER VRTDSTGSAV STDDSEQEVD IEGMEFGPGE LDSVGSSSDA DDHYSLQSGT GGDSGFGPHC RRLGRPALS
Research Areas
Citations (0)

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This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.


c-Myc creates an activation loop by transcriptionally repressing its own functional inhibitor, hMad4, in young fibroblasts, a loop lost in replicatively senescent fibroblasts. Marcotte R, et al. J Cell Biochem, 2005 Dec 1. PMID 16167342.
Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin. Pope SN, et al. J Steroid Biochem Mol Biol, 2005 Feb. PMID 15862967.
Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Hillier LW, et al. Nature, 2005 Apr 7. PMID 15815621.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Human liver specific transcriptional factor TCP10L binds to MAD4. Jiang DJ, et al. J Biochem Mol Biol, 2004 Jul 31. PMID 15469726.

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$ 368.00
Cat# AF1645a
Availability: 7-10 days
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