|Application ||WB, E|
|Other Accession||NP_036458, 22924, 100732 (mouse)|
|Calculated MW||31982 Da|
|Other Names||Microtubule-associated protein RP/EB family member 3, EB1 protein family member 3, EBF3, End-binding protein 3, EB3, RP3, MAPRE3|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-MAPRE3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plus-end tracking protein (+TIP) that binds to the plus- end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). May play a role in cell migration (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Detected at the plus end of microtubules|
|Tissue Location||Predominantly expressed in brain and muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene.
Molecular insights into mammalian end-binding protein heterodimerization. De Groot CO, et al. J Biol Chem, 2010 Feb 19. PMID 20008324.
Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. Ban R, et al. J Biol Chem, 2009 Oct 9. PMID 19696028.
Mammalian end binding proteins control persistent microtubule growth. Komarova Y, et al. J Cell Biol, 2009 Mar 9. PMID 19255245.
p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway. Hsieh PC, et al. Oncogene, 2007 Jul 26. PMID 17310996.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
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