|Application ||IHC, E, IEM|
|Other Accession||NP_060281, 54926, 67615 (mouse)|
|Calculated MW||27166 Da|
|Other Names||Ubiquitin-conjugating enzyme E2 R2, 22.214.171.124, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, UBE2R2, CDC34B, UBC3B|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBC3B Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.|
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Provided below are standard protocols that you may find useful for product applications.
CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Semplici F, Meggio F, Pinna LA, Oliviero S. Oncogene. 2002 Jun 6;21(25):3978-87. PMID: 12037680
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