|Application ||IHC, E|
|Other Accession||NP_056989.2, NP_003843.3, 8805|
|Calculated MW||116831 Da|
|Other Names||Transcription intermediary factor 1-alpha, TIF1-alpha, 6.3.2.-, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, Tripartite motif-containing protein 24, TRIM24, RNF82, TIF1, TIF1A|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TIF1A / TRIM24 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||RNF82, TIF1, TIF1A|
|Function||Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Differential interaction of nuclear receptors with the putative human transcriptional coactivator hTIF1. Thenot S, Henriquet C, Rochefort H, Cavailles V. J Biol Chem. 1997 May 2;272(18):12062-8. PMID: 9115274
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