|Application ||IHC, E|
|Other Accession||NP_065937.1, 57646|
|Calculated MW||122491 Da|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 28, 184.108.40.206, Deubiquitinating enzyme 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28, USP28, KIAA1515|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USP28 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).|
|Cellular Location||Nucleus, nucleoplasm|
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Provided below are standard protocols that you may find useful for product applications.
Fbw7 and Usp28 regulate myc protein stability in response to DNA damage. Popov N, Herold S, Llamazares M, Schülein C, Eilers M. Cell Cycle. 2007 Aug;6(19):2327-31. Epub 2007 Jul 26 PMID: 17873522
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