|Other Accession||NP_005328.2, 3071, 105855 (mouse), 315348 (rat)|
|Predicted||Human, Mouse, Rat, Dog, Horse|
|Calculated MW||128153 Da|
|Other Names||Nck-associated protein 1-like, Hematopoietic protein 1, Membrane-associated protein HEM-1, NCKAP1L, HEM1|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HEM1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F- actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE- independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648).|
|Cellular Location||Cell membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm Note=Localizes to the leading edge of polarized neutrophils|
|Tissue Location||Expressed only in cells of hematopoietic origin (PubMed:7643388, PubMed:1932118). Expressed in neutrophils (at protein level) (PubMed:16417406)|
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Provided below are standard protocols that you may find useful for product applications.
Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. PLoS Biol. 2006 Feb;4(2):e38. Epub 2006 Jan 24. PMID: 16417406
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