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HEM1 Antibody (internal region)

Peptide-affinity purified goat antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55160
Other Accession NP_005328.2, 3071, 105855 (mouse), 315348 (rat)
Predicted Human, Mouse, Rat, Dog, Horse
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 128153 Da
Additional Information
Gene ID 3071
Other Names Nck-associated protein 1-like, Hematopoietic protein 1, Membrane-associated protein HEM-1, NCKAP1L, HEM1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHEM1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCKAP1L (HGNC:4862)
Function Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F- actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE- independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648).
Cellular Location Cell membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm Note=Localizes to the leading edge of polarized neutrophils
Tissue Location Expressed only in cells of hematopoietic origin (PubMed:7643388, PubMed:1932118). Expressed in neutrophils (at protein level) (PubMed:16417406)
Research Areas
Citations (0)

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Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. PLoS Biol. 2006 Feb;4(2):e38. Epub 2006 Jan 24. PMID: 16417406

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$ 358.00
Cat# AF2836a
Availability: 7-10 days
Bulk Size
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