|Application ||WB, E|
|Other Accession||NP_001034200.1, 131405, 636331 (mouse), 301042 (rat)|
|Predicted||Human, Rat, Dog, Cow|
|Calculated MW||93385 Da|
|Other Names||E3 ubiquitin-protein ligase TRIM71, 6.3.2.-, Protein lin-41 homolog, Tripartite motif-containing protein 71, TRIM71, LIN41|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRIM71 (aa409-419) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (Probable). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A (By similarity). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (PubMed:23125361). Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity). Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (PubMed:28431233).|
|Cellular Location||Cytoplasm, P-body|
|Tissue Location||Specifically expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. Lin YC, Hsieh LC, Kuo MW, Yu J, Kuo HH, Lo WL, Lin RJ, Yu AL, Li WH, Molecular biology and evolution 2007 Nov 24 (11): 2525-34. PMID: 17890240
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