|Application ||WB, E|
|Other Accession||NP_006316.1, 5901, 19384 (mouse), 84509 (rat)|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||24423 Da|
|Other Names||GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAN (aa 197-210) Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed:10400640, PubMed:8276887, PubMed:8896452, PubMed:8636225, PubMed:8692944, PubMed:9351834, PubMed:9428644, PubMed:9822603, PubMed:26272610). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PubMed:7819259, PubMed:8896452, PubMed:8636225, PubMed:8692944, PubMed:9351834, PubMed:9428644, PubMed:9822603, PubMed:29040603, PubMed:11336674, PubMed:26272610). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PubMed:8896452, PubMed:9351834, PubMed:9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PubMed:10408446, PubMed:29040603). Required for normal progress through mitosis (PubMed:8421051, PubMed:12194828, PubMed:29040603). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed:18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed:10400640).|
|Cellular Location||Nucleus. Nucleus envelope Cytoplasm, cytosol. Cytoplasm. Melanosome. Note=Predominantly nuclear during interphase (PubMed:8421051, PubMed:12194828, PubMed:10679025) Becomes dispersed throughout the cytoplasm during mitosis (PubMed:8421051, PubMed:12194828). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).|
|Tissue Location||Expressed in a variety of tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation. Ly TK, Wang J, Pereira R, Rojas KS, Peng X, Feng Q, Cerione RA, Wilson KF, The Journal of biological chemistry 2010 Feb 285 (8): 5815-26. PMID: 20028979
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