|Application ||WB, E|
|Other Accession||NP_001035374.2, NP_001035375.2, 64282, 214627 (mouse), 307745 (rat)|
|Calculated MW||63267 Da|
|Other Names||Non-canonical poly(A) RNA polymerase PAPD5, 126.96.36.199, PAP-associated domain-containing protein 5, Terminal uridylyltransferase 3, TUTase 3, Topoisomerase-related function protein 4-2, TRF4-2, PAPD5|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAPD5 (aa497-510) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post- transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'- to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037).|
|Cellular Location||Nucleus. Nucleus, nucleolus Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165)|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_001035374.2; NP_001035375.2).
The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin. Walowsky C, Fitzhugh DJ, Castaño IB, Ju JY, Levin NA, Christman MF. J Biol Chem. 1999 Mar 12;274(11):7302-8. PMID: 10066793
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