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PAPD5 (aa497-510) Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - PAPD5 (aa497-510) Antibody (internal region) AF3550a
    AF3550a (0.2 µg/ml) staining of Mouse Spleen lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NDF8
Other Accession NP_001035374.2, NP_001035375.2, 64282, 214627 (mouse), 307745 (rat)
Reactivity Mouse, Rat
Predicted Human, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 63267 Da
Additional Information
Gene ID 64282
Other Names Non-canonical poly(A) RNA polymerase PAPD5,, PAP-associated domain-containing protein 5, Terminal uridylyltransferase 3, TUTase 3, Topoisomerase-related function protein 4-2, TRF4-2, PAPD5
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAPD5 (aa497-510) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TENT4B (HGNC:30758)
Function Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post- transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'- to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037).
Cellular Location Nucleus. Nucleus, nucleolus Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165)
Research Areas
Citations (0)

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This antibody is expected to recognize both reported isoforms (NP_001035374.2; NP_001035375.2).


The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin. Walowsky C, Fitzhugh DJ, Castaño IB, Ju JY, Levin NA, Christman MF. J Biol Chem. 1999 Mar 12;274(11):7302-8. PMID: 10066793

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$ 368.00
Cat# AF3550a
Availability: 7-10 days
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