|Application ||WB, E|
|Other Accession||NP_074036.1, NP_001746.1, 865, 12400 (mouse)|
|Calculated MW||21508 Da|
|Other Names||Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBFB|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBFB Antibody (internal region, near C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA- binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize reported isoform 1 (NP_074036.1) only, however it is expected to recognize reported mouse isoforms 1 (NP_071704.3), 2 (NP_001154928.1) and 3 (NP_001154929.1).
Vif proteins of human and simian immunodeficiency viruses require cellular CBF? to degrade APOBEC3 restriction factors. Hultquist JF, Binka M, LaRue RS, Simon V, Harris RS. Journal of virology 2012 Mar 86 (5): 2874-7. PMID: 22205746
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