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TRIC-A Antibody

Affinity purified polyclonal antibody

  • WB - TRIC-A Antibody AG1006-050
    Western blot analysis of rat brain lysate (lanes 1 and 4) and mouse brain membrane (lanes 2 and 5) and skeletal muscle (lanes 3 and 6):

    1-3. Anti-TRIC-A antibody (#AG1006), (1:200).
    4-6. Anti-TRIC-A antibody, preincubated with the control peptide antigen.

  • IHC - TRIC-A Antibody AG1006-050
    Expression of TRIC-A in rat skeletal muscle Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-TRIC-A antibody (#AG1006), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. TRIC-A labeling (red) appears in the edges of the muscle fibers, where the endomysium is present. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
  • ICC - TRIC-A Antibody AG1006-050
    Expression of TRIC-A in mouse muscle myoblast (C2C12) cell line Immunocytochemical staining of mouse paraformaldehyde-fixed and permeabilized muscle myoblast (C2C12) cell line. A. Cells were stained with Anti-TRIC-A antibody (#AG1006), (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A6ZIQ8
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 33276 Da
Homology Mouse - identical; human- will not recognize human TRIC-A.
Additional Information
Gene ID 306327
Other Names Trimeric intracellular cation channel type A, TRIC-A, TRICA, 27 kDa sarcoplasmic reticulum protein, SPR-27, Transmembrane protein 38A, Tmem38a {ECO:0000250|UniProtKB:Q3TMP8}
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DNHGAPHGMGLGTQHS, corresponding to amino acid residues 259-274 of rat (Accession A6ZIQ8). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.9 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell signaling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic /sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential1,2. Trimeric intracellular cation-specific (TRIC) channels are critical for proper management of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ 3. Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a luminal N-terminus. Functional entities are formed by homotrimerizarion4. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms3. Knock out studies of these channels have shown that TRIC-A knock mice are viable while those of TRIC-B die at the neonatal stage5. TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat TRIC-A. Anti-TRIC-A antibody (#AG1006) can be used in western blot analysis and immunocytochemistry applications, and has been designed to recognize TRIC-A from rat and mouse samples.


References 1. Weisleder, N. et al. (2008) Cell Calcium 43, 1. 2. Zhao, X. et al. (2010) J. Biol. Chem. 285, 37370. 3. Silverio, A.L.F. and Saier Jr., M.H. (2011) J. Memb. Biol. 241, 77. 4. Yazawa, M. et al. (2007) Nature 448, 78. 5. Yamazaki, D. et al. (2009) Pharmacol. Ther. 121, 265.

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$ 367.00
$ 475.00
Cat# AG1006-050
Availability: 2-3 weeks
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