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Sphingosine 1-Phosphate Receptor 2 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Sphingosine 1-Phosphate Receptor 2 Antibody AG1014-025
    Western blot analysis of rat kidney (lanes 1 and 3) and rat heart (lanes 2 and 4) lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#AG1014), (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 2 antibody, preincubated with the control peptide antigen.
  • IHC - Sphingosine 1-Phosphate Receptor 2 Antibody AG1014-025
    Expression of Sphingosine 1-Phosphate Receptor 2 in rat lung Immunohistochemical staining of paraffin embedded rat lung sections using Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#AG1014), (1:100). Sphingosine 1-Phosphate Receptor 2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52592
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 38829 Da
Homology Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical.
Additional Information
Gene ID 14739
Other Names Sphingosine 1-phosphate receptor 2, S1P receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, Sphingosine 1-phosphate receptor Edg-5, S1P receptor Edg-5, S1pr2, Edg5, Gpcr13, Lpb2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2), (Accession P52592). 2nd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.95 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (SIP), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3. Sphingosine 1-Phosphate Receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse Sphingosine 1-Phosphate Receptor 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#AG1014) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize Sphingosine 1-Phosphate Receptor 2 from mouse, rat and human samples.


1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
2. Hla, T. et al. (2001) Science 294, 1875.
3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
4. Rosen, H. et al (2009) Annu. Rev. Biochem. 78, 743.
5. Rosen, H and Liao, J. (2002) Biochim. Biophys. Acta. 1582, 222.

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$ 247.00
$ 367.00
$ 475.00
Cat# AG1014-025
Availability: 2-3 weeks
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