|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||227367 Da|
|Homology||Mouse- identical; human - 17/19 amino acid residues identical.|
|Other Names||Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav15, Scn5a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 ofֲ rat NaV1.5 (accession number P15389). Intracellular loop between domains I and II.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse muscle lysate (see Boyer, J.G. et al. (2013) in Product Citations). - Mouse heart lysate (1:200) (see Watanabe, H. et al. (2011) in Product Citations). Immunoprecipitation (IP): - Mouse cardiac myocytes (1:500) (see Malhotra, J.D. et al. (2001) in Product Citations). Immunocytochemistry (IC): - Neonatal rat ventricular cardiomyocytes (NRCs) (1:200) (see Casini, S. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1 Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias. The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.5.2 The putative structure of NaV1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.
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