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Ryanodine Receptor 1 Antibody

Affinity purified polyclonal antibody

  • WB - Ryanodine Receptor 1 Antibody AG1039-050
    Western blot analysis of rat skeletal muscle lysates:
    1. Anti-Ryanodine Receptor 1 antibody (#AG1039), (1:200).   
    2. Anti-Ryanodine Receptor 1 antibody, preincubated with the control peptide antigen.
  • IHC - Ryanodine Receptor 1 Antibody AG1039-050
    Expression of Ryanodine Receptor 1 in rat skeletal muscle
    Immunohistochemical staining of paraffin embedded sections of rat quadriceps using Anti-Ryanodine Receptor 1 antibody (#AG1039), (1:100). Ryanodine Receptor 1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue. Hematoxilin is used as the counterstain.
  • IHC - Ryanodine Receptor 1 Antibody AG1039-050
    Expression of Ryanodine Receptor 1 in mouse cerebellum Immunohistochemical staining of mouse cerebellum frozen sections using Anti-Ryanodine Receptor 1 antibody (#AG1039), (1:100), (green). A. The highest expression of Ryanodine Receptor 1 is in the molecular layer (Asterisk). B. In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 1 is localized surrounding the dendritic tree of Purkinje cells. DAPI is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21817
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 565176 Da
Homology Rat, mouse - identical.
Additional Information
Gene ID 6261
Other Names Ryanodine receptor 1, RYR-1, RyR1, Skeletal muscle calcium release channel, Skeletal muscle ryanodine receptor, Skeletal muscle-type ryanodine receptor, Type 1 ryanodine receptor, RYR1, RYDR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human Ryanodine Receptor 1 (Accession ֲ P21817).ֲ ֲ Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3 The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel . They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.   The RyR1 is expressed predominantly in skeletal muscles and areas of the brain; the human RyR1 has at least three known alternative spliced variants.5   Several diseases are attributed to mutations in RyR1 gene: Central Core Disease (CCD), multi-minicore disease (MmD) and Malignant Hyperthermia (MH).5


References 1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. 2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. 3. Hamilton, S.L. (2005) Cell Calcium 38, 253. 4. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893. 5. UniProtKB/Swiss-Prot entry P21817 [RYR1_HUMAN] Ryanodine receptor 1.

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$ 367.00
$ 475.00
Cat# AG1039-050
Availability: 2-3 weeks
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