|Calculated MW||28421 Da|
|Homology||Mouse - identical; human - 10/16 amino acid residues identical.|
|Other Names||Aquaporin-5, AQP-5, Aqp5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5ֲ ֲ (SwissProt accession number P47864 ). Intracellular, C-terminus.ֲ ֲ|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.4 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The importance of the Aquaporin water channels was underscored by awarding the 2003 Nobel Prize in Chemistry to Peter Agre “for the discovery of water channels”. Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N and C-termini. Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.
References 1. Agre, P. et al. (2002) J. Physiol. 542.1, 3. 2. Towne, J.E. et al. (2000) Am. J. Respir. Cell Mol. Biol. 22, 34. 3. Yang, F. et al. (2002) J. Biol. Chem. 278, 32173.
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