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Aquaporin 5 Antibody

Affinity purified polyclonal antibody

  • WB - Aquaporin 5 Antibody AG1045-025
    Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4): 1, 2. Anti-Aquaporin 5 antibody (#AG1045), (1:200).
    3, 4. Anti-Aquaporin 5 antibody, preincubated with the control peptide antigen.
  • IHC - Aquaporin 5 Antibody AG1045-025
    Expression of Aquaporin 5 in rat lung Immunohistochemical staining of rat lung sections using Anti-Aquaporin 5  antibody (#AG1045), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue.  A negative control is shown in the right panel.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P47864
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28421 Da
Homology Mouse - identical; human - 10/16 amino acid residues identical.
Additional Information
Gene ID 25241
Other Names Aquaporin-5, AQP-5, Aqp5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5ֲ ֲ (SwissProt accession number P47864 ). Intracellular, C-terminus.ֲ ֲ 
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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The importance of the Aquaporin water channels was underscored by awarding  the 2003 Nobel Prize in Chemistry to Peter Agre “for the discovery of water channels”.  Today, eleven mammalian proteins that belong to the Aquaporin family have been identified.  The proteins present a conserved structure of six transmembrane domains with intracellular N and C-termini.  Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.  


References 1. Agre, P. et al. (2002) J. Physiol. 542.1, 3.  2. Towne, J.E. et al. (2000) Am. J. Respir. Cell Mol. Biol. 22, 34. 3. Yang, F. et al. (2002) J. Biol. Chem. 278, 32173.

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$ 247.00
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Cat# AG1045-025
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