|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||31384 Da|
|Homology||Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical.|
|Other Names||Aquaporin-3, AQP-3, 314 kDa water channel protein, Aquaglyceroporin-3, Aqp3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accessionֲ P47862).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunoprecipitation (IP): - Mouse epidermal keratinocytes (1 μg per ml) (see Zheng, X. and Bollinger Bollag, W. (2003) in Product Citations). Indirect flow cytometry (IFC): - Human airway epithelial cell (see Avril-Delplanque, A. et al. (2005) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.4 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1 AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3
References 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 2. Hara, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7360. 3. Hara-Chikuma, M. and Verkman, A.S. (2008) Mol. Cell. Biol. 28, 326.
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