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>   home   >   Products   >   Primary Antibodies   >   Parathyroid Hormone 1 Receptor (extracellular) Antibody   

Parathyroid Hormone 1 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Parathyroid Hormone 1 Receptor (extracellular) Antibody AG1054-050
    Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates: 1-3. Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody (#AG1054), (1:200).
    4-6. Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody preincubated with the control peptide antigen.
  • IHC - Parathyroid Hormone 1 Receptor (extracellular) Antibody AG1054-050
    Expression of Parathyroid hormone receptor 1 in rat brain Immunohistochemical staining of rat dorsal Raphe nucleus using Anti-Parathyroid Hormone Receptor 1 (extracellular) antibody (#AG1054). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.
  • IHC - Parathyroid Hormone 1 Receptor (extracellular) Antibody AG1054-050
    Immuno-colocalization of Parathyroid Hormone 1 Receptor and Orexin Receptor 1 in rat ventromedial hypothalamus Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO-488 antibody (#AOR-001-AG), (1:60) and Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody (#AG1054), (1:60). A. Sections were incubated Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO-488 antibody, (green). C. Merge of A and B shows staining of Orexin Receptor 1 in nerve fibers (vertical arrows) while Parathyroid Hormone 1 Receptor is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25961
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 66261 Da
Homology Mouse - identical; human - 14/15 amino acid residues identical.
Additional Information
Gene ID 56813
Other Names Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor, PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTH1 receptor, Pth1r, Pthr, Pthr1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat Parathyroid Hormone 1 Receptor (Accession P25961 ). 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).   PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.   PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen’s disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat PTHR1. Anti-Parathyroid Hormone 1 Receptor (extracellular) antibody (#AG1054) can be used in western blot analysis. It has been designed to recognize PTHR1 from human, mouse and rat samples.


References 1. Potts, J.T. and Gardella, T.J. (2007) Ann. N.Y. Acad. Sci. 1117, 196. 2. Vilardaga, J.P. et al. (2011) Cell. Mol. Life Sci. 68, 1. 3. Potts, J.T. (2005) J. Endocrinol. 185, 311. 4. Chung, U.L. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 13030. 5. Vortkamp, A. et al. (1996) Science 273, 613. 6. Mahon, M.J. et al. (2006) Mol. Endocrinol. 20, 136. 7. Singh, A.T. et al. (2005) Endocrinology 146, 2171. 8. Schipani, E. et al. (1995) Science 268, 98. 9. Pastepe, M. et al. (2004) J. Clin. Endocrinol. Metab. 89, 3595. 10. Duchatelet, S. et al. (2005) Hum. Mol. Genet. 14, 1.

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$ 367.00
$ 475.00
Cat# AG1054-050
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