|Calculated MW||44507 Da|
|Homology||Mouse - 12/15 amino acid residues identical.|
|Other Names||Prokineticin receptor 1, PK-R1, G-protein coupled receptor 73, G-protein coupled receptor ZAQ, Prokr1, Gpr73, Pkr1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat Prokineticin receptor 1 (Accession Q8R416). Extracellular, N-terminus.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation. The biological effects of the PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin Receptor 1 (PKR1) and Prokineticin Receptor 2 (PKR2) that belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1 PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described. 1 PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain particularly in olfactory regions as well as in dorsal root ganglion (DRG) neurons. The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2 In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat PKR1 receptor. The epitope is specific for PKR1 and will not cross-react with the closely related PKR2 receptor. Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056) can be used in Western blot analysis, immunohistochemical and flow cytometry applications, and recognizes PKR1 from rat and mouse samples.
References 1. Zhou, Q-Y. (2006) Mol. Interv. 6, 330. 2. Negri, L. et al. (2006) J. Neurosci. 26, 6716. 3. Dorsch, M. et al. (2005) J. Leucoc. Biol. 78, 426.
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