|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42137 Da|
|Homology||Mouse - 18/19 amino acidresidues identical; human - 17/19 amino acid residues identical.|
|Other Names||P2Y purinoceptor 2, P2Y2, ATP receptor, P2U purinoceptor 1, P2U1, Purinergic receptor, P2ry2, P2ru1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2Y2. (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1 The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4 P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 P2Y2 receptor has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6
References 1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841. 2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. 3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. 4. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. 5. Di Virgilio, F. et al. (2001) Blood 97, 587. 6. Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther. 302, 871.
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