|Application ||WB, IHC|
|Calculated MW||40561 Da|
|Homology||Rat - 13/17 amino acid residues identical.|
|Other Names||Delta-type opioid receptor, D-OR-1, DOR-1, K56, MSL-2, Oprd1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ELVPSARAELQSSPLVN corresponding to amino acid residues 2-18 of mouse ־´-Opioid receptor (Accession P32300). Extracellular, N-terminus,.Will not recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Application Details||Immunocytochemistry (IC): - Rat neonatal adrenomedullary chromaffin cells (AMCs) (see Salman, S. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor.1 Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors.2 The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.1 All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-opioid receptor, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level.3 The opioid receptors also mediate the mood-altering properties of opioids.4 Cross-talk between m- and d- opioid receptors was demonstrated when subeffective doses of d-opioid receptors agonists modulated m-mediated analgesia.5 The d receptors are discretely distributed in the central nervous system (CNS), with a prominent gradient of receptor density from high levels in forebrain structures to relatively low levels in most hindbrain regions.2
1. Pan, L. et al. (2005) Neuroscience 133, 209.
3. Matthes, H.W. et al. (1998) J. Neurosci. 18, 7285.
4. Di Chiara, G. and North, R.A. (1992) Trends Pharmacol. Sci. 13, 185.
5. Vaught, J.L. et al. (1982) Life Sci. 30, 1443.
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