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Neurotensin Receptor 1 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Neurotensin Receptor 1 (extracellular) Antibody AG1174-050
    Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates: 1-3. Anti-Neurotensin Receptor 1 (extracellular) antibody (#AG1174), (1:200). 4-6. Anti-Neurotensin Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Neurotensin Receptor 1 (extracellular) Antibody AG1174-050
    Expression of Neurotensin Receptor 1 in human breast cancer Immunohistochemical staining of human breast cancer cells using Anti-Neurotensin Receptor 1 (extracellular) antibody (#AG1174), (1:100). Staining (brown color) is specific for epithelium-derivated malignant cells. Hematoxilin is used as the counterstain.
  • ICC - Neurotensin Receptor 1 (extracellular) Antibody AG1174-050
    Expression of Neurotensin Receptor 1 in a human breast adenocarcinoma cell line Immunocytochemical staining of a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-Neurotensin Receptor 1 (extracellular) antibody (#AG1174), (1:20), followed by Alexa 488-conjugated goat anti-rabbit antibody (green staining). B. Live view of the cells. C. Merge of (A) and (B).
  • IF - Neurotensin Receptor 1 (extracellular) Antibody AG1174-050
    Indirect flow cytometry analysis in live intact human promyelocytic leukemia HL-60 cell line:
    Black: Cells + FITC-conjugated goat anti rabbit antibody.
    Green: Cells + Anti-Neurotensin Receptor 1 (extracellular) antibody (#AG1174), (1:20) + FITC-conjugated goat-anti-rabbit antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30989
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 46259 Da
Homology Mouse - 14/16 amino acid residues identical; rat - 12/16 amino acid residues identical.
Additional Information
Gene ID 4923
Other Names Neurotensin receptor type 1, NT-R-1, NTR1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTSR1, NTRR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human Neurotensin Receptor 1 (Accession P30989). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Neurotensin receptor 1 (NTS1) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract. NTS1 and NTS2 belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily while the third neurotensin receptor NTS3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTS1 and NTS3 bind neurotensin with high affinity while NTS2 binds it with low affinity.1 NTS1 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization, although coupling with Gi/o and to Gs has been observed in some expression systems. NTS1 is mainly expressed in the brain and gastrointestinal tract. In the brain, NTS1 mediates body temperature regulation, feeding behavior and locomotion regulation. One of the best studied roles of NTS1 is the regulation of dopamine release suggesting that NTS1 can be a suitable target for the development of a new class of anti-psychotics.2 In the periphery, NTS1 expression has been linked to the control of intestine motility and in the stimulation of growth of the intestine mucosa in both physiological and pathological conditions. NTS1 has been shown to be upregulated in several human cancers where it stimulates tumor growth in response to locally produced neurotensin peptide.3 Abgent is pleased to offer a highly specific antibody directed against an epitope at the extracellular 2nd loop of the human NTS1. Anti-Neurotensin Receptor-1 (extracellular) antibody (#AG1174) can be used in Western blot analysis, immunohistochemical, immunocytochemical, and flow cytometry applications.


1. Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302.
2. Binder, E.B. et al. (2001) Pharmacol. Rev. 53, 453.
3. Souaz, F. et al. (2006) Cancer Res. 66, 6243.

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$ 367.00
$ 475.00
Cat# AG1174-050
Availability: 2-3 weeks
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