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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Rat H2 Histamine Receptor (extracellular) Antibody   

Rat H2 Histamine Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Rat H2 Histamine Receptor (extracellular) Antibody AG1226-050
    Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): 1, 2. Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AG1226), (1:200). 3, 4. Anti- Rat H2 Histamine Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Rat H2 Histamine Receptor (extracellular) Antibody AG1226-050
    Expression of H2 Histamine Receptor in rat stomach Immunohistochemical staining of paraffin embedded section of rat stomach using Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AG1226), (1:100). H2R (brown staining) is expressed in parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
  • IF - Rat H2 Histamine Receptor (extracellular) Antibody AG1226-050
    Indirect flow cytometery analysis in live intact RBL (rat basophilic leukemia) cell lines:
    Black: Unstained cells + goat-anti-rabbit-FITC.
    Green: Cells+ Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AG1226), (1:20) + goat-anti-rabbit-FITC.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25102
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 40253 Da
Homology Mouse - identical.
Additional Information
Gene ID 25461
Other Names Histamine H2 receptor, H2R, HH2R, Gastric receptor I, Hrh2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat H2 Histamine Receptor (Accession P25102). 2nd extracellular loop.Will not recognize human H2 Histamine Receptor.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including brain, gastrointestinal tract and heart. The most studied physiological role of the H2 receptors is their involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia. Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat H2 Histamine receptor. Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AG1226) can be used in Western blot analysis, indirect flow cytometry and immunohistochemical applications, and recognizes H2 Histamine receptor from rat and mouse samples.


1. Hill, S.J., (1997) Pharmacol. Rev., 49, 253.
2. Del Valle, J. and Gantz, I. (1997) Am. J. Physiol. Gastrointest. Liver Physiol., 273, 987.
3. Kobayashi, T. et al. (2000) J. Clin Invest., 105, 1741.

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$ 367.00
$ 475.00
Cat# AG1226-050
Availability: 2-3 weeks
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