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H1 Histamine Receptor Antibody

Affinity purified rabbit polyclonal antibody

  • WB - H1 Histamine Receptor Antibody AG1227-050
    Western blot analysis of rat heart membranes (lanes 1 and 3) and rat basophilic leukemia (RBL) cell lysates (lanes 2 and 4): 1, 2. Anti-H1 Histamine Receptor antibody (#AG1227), (1:200). 3, 4. Anti-H1 Histamine Receptor antibody, preincubated with the control peptide antigen.
  • WB - H1 Histamine Receptor Antibody AG1227-050
    Western blot analysis of mouse brain membranes: 1. Anti-H1 Histamine Receptor antibody (#AG1227), (1:400). 2. Anti-H1 Histamine Receptor antibody, preincubated with the control peptide antigen.
  •  - H1 Histamine Receptor Antibody AG1227-050
    Expression of H1 Histamine receptor in mouse brain Immunohistochemical staining of mouse ventromedial hypothalamus (VMH) using Anti-H1 Histamine Receptor antibody (#AG1227). A. H1 histamine receptor (green fluorescence) appears in the outline of the VMH nucleus (arrows). B. DAPI (blue) counterstain labels all cell nuclei including VMH.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31390
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 55693 Da
Homology Mouse - identical; human, dog - 13/14 amino acid residues identical.
Additional Information
Gene ID 24448
Other Names Histamine H1 receptor, H1R, HH1R, Hrh1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat H1 Histamine Receptor (Accession P31390). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1 H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1 receptors are widely distributed through the brain where the mediate sleep-wake cycles and regulate cognitive functions. H1 receptors are expressed in a several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles. 2,3 H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular 3rd loop of the rat H1 Histamine receptor. Anti-H1 Histamine Receptor antibody (#AG1227) can be used in Western blot analysis and immunohistochemical applications, and recognizes H1 Histamine receptor from rat, mouse and human samples.


1. Hill, S.J., (1997) Pharmacol. Rev., 49, 253
2. Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
3. Haas, H.L. et al. (2008) Physiol. Rev., 88, 1183.

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$ 367.00
$ 475.00
Cat# AG1227-050
Availability: 2-3 weeks
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