|Application ||WB, IHC|
|Calculated MW||37748 Da|
|Homology||Mouse - identical.|
|Other Names||Gonadotropin-releasing hormone receptor, GnRH receptor, GnRH-R, Gnrhr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRH Receptor (Accession P30969). Extracellular, N-terminus.Not recommended for use with human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Gonadotropin-releasing hormone (GnRH) or Luteinizing hormone releasing hormone (LHRH) is a decapeptide synthesized and released from neurons in the hypothalamus and stimulates the synthesis and release of gonadotropin hormone (or luteinizing hormone) and follicle stimulating hormone from the pituitary gland1. GnRH is expressed as various variants, and most vertebrates express more than one form of GnRH1-3. GnRH and its variants bind and activate GnRH receptor (GnRHR), a member of the G-protein coupled receptor (GPCR) superfamily. Three forms of the receptor have been identified in vertebrates (types I, II and III). Like all members, GnRHR has seven transmembrane domains and an extracellular N-terminus and an intracellular C-terminal tail. The receptor also has a number of glycosylation sites in its extracellular loops. In addition, the C-terminal tail is unusually short and lacks consensus sites known to undergo post-translational modifications like phosphorylation. In consequence, this affects the receptor’s desensitization and internalization4. Expression of GnRHR is detected in the gonadotrope and various tissues like breast, gonads, prostate and uterus4,5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GnRH receptor. Anti-GnRH Receptor (extracellular) antibody (#AG1236) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GnRHR from mouse and rat samples.
1. Naor, Z. (2009) Front. Neuroendocrinol. 30, 10.
2. Millar, R.P. et al. (2004) Endocr. Rev. 25, 235.
3. Tello, J.A. et al. (2005) Endocrinology 146, 4061.
4. Bliss, S.P. et al. (2010) Front. Neuroencrinol. 31, 322.
5. Cheung, L.W. and Wong, A.S. (2008) FEBS J. 275, 5479.
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