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Galanin Receptor Type 2 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Galanin Receptor Type 2 Antibody AG1240-050
    Western blot analysis of rat hippocampus, DRG (lanes 2 and 5), brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates: 1, 2, 3, 7, 8. Anti-Galanin Receptor Type 2 antibody (#AG1240), (1:200). 4, 5, 6, 9, 10. Anti-Galanin Receptor Type 2 antibody, preincubated with the control peptide antigen
  • IHC - Galanin Receptor Type 2 Antibody AG1240-050
    Expression of GALR2 in rat DRG Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Galanin Receptor Type 2 antibody (#AG1240), (1:100). GALR2 is expressed in the DRG neurons. Hoechst 33342 is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O88854
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 40516 Da
Homology Rat - identical; human - 11/13 amino acid residues identical.
Additional Information
Gene ID 14428
Other Names Galanin receptor type 2, GAL2-R, GALR-2, Galr2, Galnr2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2 (Accession O88854). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin Receptors, the Galanin Receptor Type 1 (GALR1), Galanin Receptor Type 2 (GALR2) and Galanin Receptor Type 3 (GALR3). The Galanin Receptors belong to the G-Protein Coupled Receptor superfamily.1-4 GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. GALR2 high levels of expression were also found in rat dorsal root ganglia.1 GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse Galanin Receptor type 2 (GALR2). Anti-Galanin Receptor Type 2 antibody (#AG1240) can be used for Western blot analysis and immunohis


1. Branchek, T.A. et al. (2000) Trends Pharmacol Sci, 21, 109.
2. Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94.
3. Barreda-Go´mez, G. et al. (2005) Neuropeptides 39, 153.
4. Lu, X. et al. (2005) Neuropeptides 39, 147.
5. Counts, S.E. et al. (2003) Mol. Interv. 3, 137.

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$ 367.00
$ 475.00
Cat# AG1240-050
Availability: 2-3 weeks
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