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>   home   >   Products   >   Primary Antibodies   >   CaV1.2 antibody Antibody   

CaV1.2 antibody Antibody

Affinity purified rabbit polyclonal antibody

  • WB - CaV1.2 antibody Antibody AG1363-025
    Western blot analysis of rat brain membranes: 1. Anti-CaV1.2 antibody (#AG1363), (1:200). 2. Anti-CaV1.2 antibody, preincubated with the control peptide antigen.
  • IHC - CaV1.2 antibody Antibody AG1363-025
    Expression of Cav1.2 in mouse cerebellum Immunohistochemical staining of mouse cerebellum with Anti-Cav1.2 antibody (#AG1363). A. Cav1.2 (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows). B. Staining of Purkinje nerve cells with mouse anti-Calbindin 28K (green) demonstrates the location of dendrites in the molecular layer. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22002
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 243482 Da
Homology Mouse - identical; guinea pig -17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical.
Additional Information
Gene ID 24239
Other Names Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Rat brain class C, RBC, Voltage-gated calcium channel subunit alpha Cav12, Cacna1c, Cach2, Cacn2, Cacnl1a1, Cchl1a1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat Cav1.2 (Accession P22002).  Intracellular loop between domains II and III.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Rat myocytes (see Li, J. et al. (2013) in Product Citations). - Rat primary submucosa cells (see Rehn, M. et al. (2013) in Product Citations). - Mouse synaptosome (1:400) (see Saggu, S. et al. (2013) in Product Citations). - Mouse mesenteric and pulmonary arteries (1:200) (see Ko, E.A. et al. (2013) in Product Citations). - HEK-293 cells expressing CaV1.2 (see Boczek, N.J. et al. (2013) in Product Citations). - Human cardiac tissue (see Crossman, D.J. et al. (2011) in Product Citations). Immunoprecipitation (IP): - CaV1.2 transfected HEK-293 cells and mouse heart lysate (see Rougier, J.S. et al. (2011) in Product Citations). Immunohistochemistry (IH): - Rat retinal sections (1:1000) (see Sargoy, A. et al. (2013) in Product Citations). - Rat intracardiac ganglia and stellate ganglia neurons (see Tu, H. et al. (2014) in Product Citations). - Rat retinal sections (1:250-1:500) (see Liu, X. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat primary submucosa cells (1:50) (see Rehn, M. et al. (2013) in Product Citations). - Rat insulinoma cells (RIN) (see Parkash, J. (2011) in Product Citations). - Mouse chromaffin cells (1:200). (see Perez-Alvarez, A. et al. (2011) in Product Citations). Indirect flow cytometry (IFC): - Rat mast cells (RBL-2H3 cells, 1μg/5x105 cells). (see Yoshimaru, T. et al. (2009) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl deionized water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).


1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337.
4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723.
5. Allard, B. et al. (2000) J. Biol. Chem. 275. 25556.

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Cat# AG1363-025
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