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Calcium Sensing Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Calcium Sensing Receptor (extracellular) Antibody AG1435-025
    Western blot analysis of Colo205 (lanes 1and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates: 1,2,5. Anti-Calcium Sensing Receptor (extracellular) antibody (#AG1435), (1:200). 3,4,6. Anti-Calcium Sensing Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - Calcium Sensing Receptor (extracellular) Antibody AG1435-025
    Expression of CaSR in C6 cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 (rat brain glioma) cells. A. Staining using Anti-Calcium Sensing Receptor (extracellular) antibody (#AG1435), (1:50), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using Hoechst 33342. C. Merged images of panels A and B.
  • FC - Calcium Sensing Receptor (extracellular) Antibody AG1435-025
    Indirect Flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells:
    Black: Unstained cells.
    Green: Cells + Anti-Calcium Sensing Receptor (extracellular) antibody (#AG1435), (10 µg/5x105 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41180
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 120675 Da
Homology Rat, mouse, bovine, canis - identical.
Additional Information
Gene ID 846
Other Names Extracellular calcium-sensing receptor, CaSR, Parathyroid cell calcium-sensing receptor 1, PCaR1, CASR, GPRC2A, PCAR1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180). N-terminus, Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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The Calcium-Sensing Receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism, such as parathyroid cells and kidney.1,2 The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3 CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4 CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5 Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas,lower levels of expression were found in compared to normal colon tissue. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope at the N-terminus of human CaSR. Anti-Calcium Sensing Receptor (extracellular) antibody (#AG1435) can be used in western blot analysis and immunocytochemistry applications, and recognizes CaSR in human, rat and mouse samples.


2. Racz, G.Z. et al. (2002) Gut 51, 705.
3. Canaff, L. and Hendy, G.N. (2002) J. Biol. Chem. 277, 30337.
4. Chen, R.A. and Goodman, W.G. (2004) Am. J. Physiol. Renal Physiol. 286, F1005
5. Chattopadhyay, N. (2006) Am. J. Physiol. Endocrinol. Metab. 290, E761.

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Cat# AG1435-025
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