|Application ||WB, IHC, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||251527 Da|
|Homology||Mouse - 16/17 amino acid residues identical; human - 10/17 amino acid residues identical.|
|Other Names||Voltage-dependent P/Q-type calcium channel subunit alpha-1A, Brain calcium channel I, BI, Calcium channel, L type, alpha-1 polypeptide, isoform 4, Rat brain class A, RBA-I, Voltage-gated calcium channel subunit alpha Cav21, Cacna1a, Cach4, Cacn3, Cacnl1a4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CaV2.1 (Accession P54282). Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 10 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Voltage dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation.1 The voltage dependent calcium channels are composed of several subunits; α1, β, α2δ and γ. Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type. The Cav2.1 (formally named α1A) makes up the α1 poreforming subunit in P/Q type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with Cav2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,2 Mutations in the Cav2.1 have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6).1,3-5 The involvement of Cav2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA (#RTA-500), a specific blocker of the Cav2.1 channel.6 The blocking sensitivity is dependent on the a subunit isoform and on the splice variant.7,8
1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
2. Scheuber, A. et al. (2004) J. Neurosci. 24,10402.
3. Ophoff, R. A. et al (1996) Cell 87, 543.
4. Ishikawa, K. et al. (1997) Am. J. Hum. Genet. 61, 336.
5. Tottene, A. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 13284.
6. Mintz, I.M. et al. (1992) Nature 355, 827.
7. Moreno, H. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 14042.
8. Bourinet, E. et al. (1999) Nat. Neurosci. 2, 407.
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