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CaV2.1 antibody Antibody

Affinity purified rabbit polyclonal antibody

  • WB - CaV2.1 antibody Antibody AG1461-025
    Western blot analysis of rat brain membranes: 1. Anti-CaV2.1antibody (#AG1461), (1:200). 2. Anti-CaV2.1 antibody, preincubated with the control peptide antigen. Human red blood cells (see Andrews, D.A. et al. (2002) Blood 100, 3392.).
  • IHC - CaV2.1 antibody Antibody AG1461-025
    Expression of Cav2.1 in mouse cerebellum Immunohistochemical staining of mouse cerebellum with Anti-Cav2.1 antibody (#AG1461), (1:100). A. Cav2.1 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54282
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 251527 Da
Homology Mouse - 16/17 amino acid residues identical; human - 10/17 amino acid residues identical.
Additional Information
Gene ID 25398
Other Names Voltage-dependent P/Q-type calcium channel subunit alpha-1A, Brain calcium channel I, BI, Calcium channel, L type, alpha-1 polypeptide, isoform 4, Rat brain class A, RBA-I, Voltage-gated calcium channel subunit alpha Cav21, Cacna1a, Cach4, Cacn3, Cacnl1a4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CaV2.1 (Accession P54282). Intracellular loop between domains II and III.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 10 µg antibody.
Citations (0)

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Voltage dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation.1 The voltage dependent calcium channels are composed of several subunits; α1, β, α2δ and γ. Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type. The Cav2.1 (formally named α1A) makes up the α1 poreforming subunit in P/Q type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with Cav2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,2 Mutations in the Cav2.1 have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6).1,3-5 The involvement of Cav2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA (#RTA-500), a specific blocker of the Cav2.1 channel.6 The blocking sensitivity is dependent on the a subunit isoform and on the splice variant.7,8


1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
2. Scheuber, A. et al. (2004) J. Neurosci. 24,10402.
3. Ophoff, R. A. et al (1996) Cell 87, 543.
4. Ishikawa, K. et al. (1997) Am. J. Hum. Genet. 61, 336.
5. Tottene, A. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 13284.
6. Mintz, I.M. et al. (1992) Nature 355, 827.
7. Moreno, H. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 14042.
8. Bourinet, E. et al. (1999) Nat. Neurosci. 2, 407.

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$ 247.00
$ 367.00
$ 475.00
Cat# AG1461-025
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