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>   home   >   Products   >   Primary Antibodies   >    Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide   

Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone TOP1MT/488 ]

  • IHC -  Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide AH11100-20
    Formalin-fixed, paraffin-embedded human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
  • IHC -  Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide AH11100-20
    Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
  • IHC -  Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide AH11100-20
    Formalin-fixed, paraffin-embedded human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969P6
Other Accession 116447, 528574
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG2b, kappa
Clone Names TOP1MT/488
Calculated MW 70kDa
Additional Information
Gene ID 116447
Other Names DNA topoisomerase I, mitochondrial, TOP1mt,, TOP1MT
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Cellular Location Mitochondrion.
Tissue Location Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
Citations (0)

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DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.


Zhang, H., Barcel�, J.M., Lee, B., Kohlhagen, G., Zimonjic, D.B., Popescu, N.C. and Pommier, Y. 2001. Human mitochondrial topoisomerase I. Proc. Natl. Acad. Sci. USA 98: 10608-10613. | Zhang, H., Meng, L.H. and Pommier, Y. 2007. Mitochondrial topoisomerases and alternative splicing of the human TOP1mt gene. Biochimie 89: 474-481

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$ 199.00
$ 429.00
Cat# AH11100-20
Availability: 3-5 days
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