|Application ||IF, FC|
|Other Accession||2214, 372679|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD16 / Fc-gamma Receptor III Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD16A, FCG3, FCGR3, IGFR3|
|Function||Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Secreted. Note=Exists also as a soluble receptor|
|Tissue Location||Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a protein of 50-65kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This MAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.
Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989. | Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106. | Nagarajan S et al. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem 1995, 270(43):25762-25770 | McKenzie SE and Schreiber AD. Biological advances and clinical applications of Fc receptors for IgG. Curr Opin Hematol 1994, 1(1):45-52. | Cerboni C et al. CD16-mediated activation of phosphatidylinositol-3 kinase (PI-3K) in human NK cells involves tyrosine phosphorylation of Cbl and its association with Grb2, Shc, pp36 and p85 PI-3K subunit. Eur J Immunol 1998, 28(3):1005-1015. | Braakman E et al. CD16 on human �T lymphocytes: expression, function, and specificity for mouse IgG isotypes. Cell Immunol 1992, 143:97-107
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