|Application ||IHC-P, IF, E|
|Isotype||Mouse / IgG2b, kappa|
|Calculated MW||48075 Da|
|Other Names||Adipophilin; ADFP; Adipose differentiation-related protein (ADRP); Perilipin-2 (PLIN2)|
|Application Note||ELISA (For coating, order Ab without BSA);,Immunofluorescence (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-Adipophilin / Perilipin-2 (Marker of Obesity) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in development and maintenance of adipose tissue.|
|Cellular Location||Membrane; Peripheral membrane protein. Lipid droplet|
|Tissue Location||Milk lipid globules.|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
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