|Application ||WB, IHC-P, IF, FC, IP|
|Isotype||Mouse / IgG1|
|Calculated MW||309265 Da|
|Other Names||Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)|
|Application Note||Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1.0ug/ml); Immunoprecipitation (0.5-1 �g/500ug protein lysate);,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.|
|Cellular Location||Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules|
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Provided below are standard protocols that you may find useful for product applications.
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
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