|Application ||WB, IHC-P, IF, FC|
|Reactivity||Human, Mouse, Rat|
|Isotype||Mouse / IgG1, kappa|
|Calculated MW||25809 Da|
|Other Names||CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28|
|Application Note||Functional Studies (Order Ab without Azide);,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1.0ug/ml for 2 hours at RT);,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-CD81 / TAPA-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein. Note=Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane|
|Tissue Location||Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
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